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1. tissue storage
1. Tissue storage
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= Impact of Fomblin on Histology =
= Packing =
= Scanning =
= Post-Processing =
= Histology =
== Hyare et al., 2008 ==
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|= Tissue Storage =|
This page is meant to be a collection of notes on Fomblin with regard to the LCN's standard operating protocol for ex vivo samples.
Background: Fomblin is the name brand of a fluoropolymer produced by Solvay Specialty Polymers S.p.A. that has been used since 1998 (Huang, G. Y., et al., 1998) as an immersion solution to improve MR-imaging SNR in tissue samples. The fluoropolymer uniquely lack's any MR-signal but has magnetic susceptibility similar to that of tissue, which results in MR-images with high SNR & CNR between the Fomblin and any suspended tissue. Since the use of Fomblin in MR-imaging is still quite young, the impact of the solution on tissue properties is unknown. We are maintaining this wiki page to document the following 5 levels of ex vivo processing with Fomblin to record any personal and literature-based experiences with the solution.
5 levels of the ex vivo procedure where Fomblin can impact tissue:
- Tissue storage
Hyare et al., 2008
The group Allison is referring to (Hyare et al., 2008) found that storing tissue in Fomblin for 2 days prior to MRI had no effect on scan or histology results when compared to matched samples stored in 10% formol-saline.
Synopsis: Formalin-fixed mouse brain tissue placed in Fomblin for 2 days prior to T1, T2, DWI, and MTR scans @ 9.4T produced the same results as formalin-fixed controls placed in 10% formol-saline solution 2 days prior to scanning. Both were scanned in Fomblin. They compared ROI-based quantitative MR measures, anatomical & cellular histology, and immunohistochemical staining.
Fluorinert has been used in a number of studies. A quick search came back with +5 exvivo studies since 2004 using Fluorinert as an in-scan solution - typically Flourinert FC-77, not FC-3283.
3M Fluorinert Electronic Liquids for Electronics: https://www.3m.com/3M/en_US/company-us/all-3m-products/~/All-3M-Products/Electronics/Chemicals/Chemicals/Fluorinert-Electronic-Liquids/?N=5002385+8709318+8710710+8711017+8736409+8745514+3294857497&rt=r3
Studies using Flourinert: Noristani et al., 2015, Colon-Perez et al., 2015, Kovačević et al., 2004, etc. (https://scholar.google.com/scholar?hl=en&as_sdt=0,22&q=ex+vivo+mri+fluorinert)