This page is meant to be a collection of notes on Fomblin with regard to the LCN's standard operating protocol for ex vivo samples.

Background: Fomblin is the name brand of a fluoropolymer produced by Solvay Specialty Polymers S.p.A. that has been used since 1998 (Huang, G. Y., et al., 1998) as an immersion solution to improve MR-imaging SNR in tissue samples. The fluoropolymer uniquely lack's any MR-signal but has magnetic susceptibility similar to that of tissue, which results in MR-images with high SNR & CNR between the Fomblin and any suspended tissue. Since the use of Fomblin in MR-imaging is still quite young, the impact of the solution on tissue properties is unknown. We are maintaining this wiki page to document the following 5 levels of ex vivo processing with Fomblin to record any personal and literature-based experiences with the solution.

5 levels of the ex vivo procedure where Fomblin can impact tissue:

  1. Packing
  2. Scanning
  3. Post-Processing
  4. Histology
  5. Tissue storage


Galden HT200

From email correspondence with Brain Edlow (03/28/17): We were initially using Fomblin LC08 from Solvay Solexis. Solvay Solexis stopped selling Fomblin LC08 a few years ago, so we transitioned to using Kurt J. Lesker products. Initially we used Fomblin 06/6 from Kurt J. Lesker. However, the specific gravity of Fomblin 06/6 was slightly higher than that of LC08, and as a result it was more technically challenging to pack whole brains in this fluid within an air-tight plastic bag for ex vivo MRI. We therefore transitioned to Galden HT200 (Galden is just a brand name of a Fomblin product) because of its lower specific gravity, which has made the brain packing process go more smoothly. I don't believe that anyone in our lab has noticed any differences in the quality of the imaging data acquired with these three slightly different fomblin solutions.




Hyare et al., 2008

Hyare et al., 2008 found that storing tissue in Fomblin for 2 days prior to MRI had no effect on scan or histology results when compared to matched samples stored in 10% formol-saline.

Synopsis: Formalin-fixed mouse brain tissue placed in Fomblin for 2 days prior to T1, T2, DWI, and MTR scans @ 9.4T produced the same results as formalin-fixed controls placed in 10% formol-saline solution 2 days prior to scanning. Both were scanned in Fomblin. They compared ROI-based quantitative MR measures, anatomical & cellular histology, and immunohistochemical staining.

Tissue Storage