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The Time Course is a binary volume with multiple time points or conditions. The data is displayed in a graph so that all time points can be seen for a given vertex. When a vertex is clicked, the corresponding voxel is found in the binary volume. TkSurfer graphs the data for all time points at the chosen vertex.
Loading Time Course Volumes Loading binary volume Overlays and Time Course data is similar. Use the File->Load Time Course... command to load a time course volume. TkSurfer requires functional volumes to be in binary volume format. It will look for a header file with the same stem as the slice data or just guess the dimensions from the bfile. You need to specify the directory of the data, the stem, and optionally, a registration file. The format of a bfile name looks like this:
/path/to/data/stem_000.bfloat BR /path/to/data/stem_000.bshort
The numbers go from 0 to the number of slices minus one. The 'stem' represents any text that appears before the underscore before the number in the bfile.
You can specify a registration file to use. If you do not, TkSurferwill look in the same directory as the bfile data for a file called register.dat. A registration file is a matrix that defines the translation between anatomical RAS space and functional RAS space. TkSurfer uses this to align the functional volume with the anatomical volume.